TAU > Faculty of Life Sciences > Dept. of Biochemistry and Molecular Biology > Laboratory of Molecular and Functional Neurobiology of Learning and Memory

Dr. Anton Sheinin

ASh Senior Scientist
Email: This email address is being protected from spambots. You need JavaScript enabled to view it. 
Phone: 972-3-6407971

*About Me        

*My Projects

*My Courses    

*My Devices

*Publications  

*CV

*ResearchGate

 

ABOUT ME

My general research interests include the mechanisms of neuronal communication, learning and memory. Specifically, I am interested in determination of the role of different neuronal proteins in modulation of synaptic transmission under physiological (i.e., aging) and pathological conditions (dementia, Alzheimer's disease, post-traumatic syndrome, etc). In my daily work, I utilize the methods of in vitro electrophysiology (patch clamp, field recordings), molecular biology and viral infection. In addition, I am interested in methods of biological signal amplification, detection and analysis, construction and modifying of electronic devices for use in electrophysiological experiments (amplifiers, stimulators, filters, perfusion controllers).

 

MY PROJECTS

  1. Aging related changes in long-term synaptic plasticity
  2. Impact of non-receptor tyrosine kinase pathway on long-term synaptic plasticity and memory formation
  3. Development of microfluidic chamber based methodology to evoke long-term synaptic plasticity in neuronal cell culture

 

MY COURSES

1. Introduction to Electrophysiological Instrumentation (syllabus)

2. Neurobiology Laboratory course (syllabus)

 

MY DEVICES

1. Electrophysiological stimulus isolator

Stim1
The electrophysiological stimulus isolator is based on circuit published by Brasil et al. Optical isolation, high (up to 200V) compliance, regulated current and low stray capacitance made this device useful for stimulation of the brain slices during the electrophysiological experiments. Performance of this isolator is comparable to many commercial units. Two units were constructed, one of them is currently used in in the laboratory of Dr. Izhak Michaelevski.

 

 

2. Headstage of the field potential amplifier

headst
The old Grass Polygraph system equipped with 7P5 AC EEG preamp could be used for field recordings from brain slices. However, the input impedance of 7P5 could be not enough for coupling with the 1-6 MOhm glass micropipettes that are commonly used for slice recordings. In order to increase the input impedance, the simple headstage and microelectrode holder were constructed. The headstage is based on TL072, dual low-noise JFET opamp. The chip is powered from Grass Polygraph power supply (+-15V). The headstage circuit is mounted inside the small metal case with a male BNC connector for the microelectrode holder. The micropipette is mounted inside the holder based on female BNC connector. This setup provides a low-cost solution for measuring field potentials from brain slices. The quality of the recordings is comparable to that of the commercial amplifiers. The system is currently used in the laboratory of Dr. Izhak Michaelevski.

 

3. Current regulator for powering the LED used in optogenetics

 20130404 144437 1

20130404 144621 1

Provides a regulated current (max 2A) for powering the high intensity blue LED used for activation of channelrhodopsin. The device could be triggered by TTL pulse and allow precise timing of the channelrhodopsin activation. This device is currently used in the laboratory of Prof. Uri Ashery.

 

  

4. Manual and computer-controlled solenoid valve perfusion system

PerfContr
The perfusion system consists of Lee Corp. solenoid valves that could be opened manually and/or TTL command. This allows precise timing of the drug application during the electrophysiological experiment. A number of systems were constructed.

 

 

5. Stepper motor controller for fast perfusion

The stepper motor and its controller provide precise, computer-controlled movement of the multi-barrel applicator against the cell in whole cell experiments in culture. Could be used for studying the kinetics of fast processes (activation and deactivation of ligand gated receptor-channels, sucrose challenge experiments, etc). This controller was used for studying NMDA receptor-channel kinetics in the laboratory of Dr. Morris Benveniste.

 

  

6. Recording bath temperature controller  

 ThermoController
The temperature controller will maintain constant temperature in the electrophysiological recording chamber, with a hysteresis of 0.1 oC.

 

 

7. StimDuino

Capture
An Arduino-controlled stimulus isolator that allows the user to manipulate the stimulation current with sufficient precision.  In addition, the intensity of the stimulation current could be programmed from the software and applied repetitively. This is particularly important for automation of the input-output experiments in some preparations, for example brain slices and in vivo electrophysiology in behaving animals in order to reduce the external influences of the researcher.

8. Electrophysiological AC amplifier

 Capture1

recordings example1

The differential AC amplifier for the in vivo experiments.   Characteristics:

Adjustable gain (x): 500, 1000, 2000, 5000

Bandwidth: 0.3 Hz – 4.1 kHz

Power supply: -9V – 0 + 9V (two 9V batteries)

 

This is a part of the setup in Dr. Inna Slutsky's lab, and the recordings are the courtesy of Ms. Tatyana Fedorova 


 

 

 

PUBLICATIONS

1: Lavi A, Sheinin A, Shapira R, Zelmanoff D, Ashery U. DOC2B and Munc13-1 Differentially Regulate Neuronal Network Activity. Cereb Cortex. 2013 Mar 28. [Epub ahead of print] PubMed PMID: 23537531.

2: Barak B, Okun E, Ben-Simon Y, Lavi A, Shapira R, Madar R, Wang Y, Norman E, Sheinin A, Pita MA, Yizhar O, Mughal MR, Stuenkel E, van Praag H, Mattson MP, Ashery U. Neuron-Specific Expression of Tomosyn1 in the Mouse Hippocampal Dentate Gyrus Impairs Spatial Learning and Memory. Neuromolecular Med. 2013 Mar 22. [Epub ahead of print] PubMed PMID: 23519441.

3: Feinshreiber L, Singer-Lahat D, Friedrich R, Matti U, Sheinin A, Yizhar O, Nachman R, Chikvashvili D, Rettig J, Ashery U, Lotan I. Non-conducting function of the Kv2.1 channel enables it to recruit vesicles for release in neuroendocrine and nerve cells. J Cell Sci. 2010 Jun 1;123(Pt 11):1940-7. doi: 10.1242/jcs.063719. PubMed PMID: 20484665.

4: Valensi-Kurtz M, Lefler S, Cohen MA, Aharonowiz M, Cohen-Kupiec R, Sheinin A, Ashery U, Reubinoff B, Weil M. Enriched population of PNS neurons derived from human embryonic stem cells as a platform for studying peripheral neuropathies. PLoS One. 2010 Feb 18;5(2):e9290. doi: 10.1371/journal.pone.0009290. PubMed PMID: 20174633; PubMed Central PMCID: PMC2823780.

5: Sheinin A, Talani G, Davis MI, Lovinger DM. Endocannabinoid- and mGluR5-dependent short-term synaptic depression in an isolated neuron/bouton preparation from the hippocampal CA1 region. J Neurophysiol. 2008 Aug;100(2):1041-52. doi: 10.1152/jn.90226.2008. Epub 2008 May 21. PubMed PMID: 18497350; PubMed Central PMCID: PMC2525720.

6: Zikich D, Mezer A, Varoqueaux F, Sheinin A, Junge HJ, Nachliel E, Melamed R, Brose N, Gutman M, Ashery U. Vesicle priming and recruitment by ubMunc13-2 are differentially regulated by calcium and calmodulin. J Neurosci. 2008 Feb 20;28(8):1949-60. doi: 10.1523/JNEUROSCI.5096-07.2008. PubMed PMID: 18287511.

7: Singer-Lahat D, Sheinin A, Chikvashvili D, Tsuk S, Greitzer D, Friedrich R, Feinshreiber L, Ashery U, Benveniste M, Levitan ES, Lotan I. K+ channel facilitation of exocytosis by dynamic interaction with syntaxin. J Neurosci. 2007 Feb 14;27(7):1651-8. PubMed PMID: 17301173.

8: Peretz A, Sheinin A, Yue C, Degani-Katzav N, Gibor G, Nachman R, Gopin A, Tam E, Shabat D, Yaari Y, Attali B. Pre- and postsynaptic activation of M-channels by a novel opener dampens neuronal firing and transmitter release. J Neurophysiol. 2007 Jan;97(1):283-95. Epub 2006 Oct 18. PubMed PMID: 17050829.

9: Sheinin A, Nahum-Levy R, Shavit S, Benveniste M. Specificity of putative partial agonist, 1-aminocyclopropanecarboxylic acid, for rat N-methyl-D-aspartate receptor subunits. Neurosci Lett. 2002 Jan 11;317(2):77-80. PubMed PMID: 11755244.

10: Sheinin A, Shavit S, Benveniste M. Subunit specificity and mechanism of action of NMDA partial agonist D-cycloserine. Neuropharmacology. 2001 Aug;41(2):151-8. PubMed PMID: 11489451.

 

Book Chapter:

Ashery U, Bielopolski N, Lavi A, Barak B, Michaeli L, Ben-Simon Y, Sheinin A, Bar-On D, Shapira Z, Gottfried I 2013: The Molecular Mechanisms Underlying Synaptic Transmission: A View of the Presynaptic Terminal. IN: The Synapse: Structure and Function. Editor: Johnson JE, Neuroscience-Net

 

CURRICULUM VITAE

A. Employment
2007 – present: Research Associate, George W. Wise School of Life Sciences, Tel-Aviv University, Israel

2006 – 2007: Visiting Postdoctoral Scientist, Laboratory for Integrative Neuroscience, National Institute on Alcoholism and Alcohol Abuse, National Institutes of Health, Bethesda, MD.

2005 – 2006: Postdoctoral Fellow, Department of Physiology and Pharmacology, Sackler School of Medicine, Tel-Aviv University, Israel.

2000 – 2004: Laboratory instructor at Department of Physiology and Pharmacology, Sackler School of Medicine, Tel-Aviv University, Israel.

 

B. Education

2000 – 2005: Ph. D., Department of Physiology and Pharmacology, Sackler School of Medicine, Tel-Aviv University, Israel.
Supervisor: Morris Benveniste, Ph. D.
Thesis: "The pre- and postsynaptic mechanisms affecting the integration of excitatory inputs: focus on NMDA receptors and M-current".

1996 – 2000: M. Sc., Department of Physiology and Pharmacology, Sackler School of Medicine, Tel-Aviv University, Israel.
Supervisor: Morris Benveniste, Ph. D.
Thesis: "Dependence of the NMDA receptor-channel activation on the inter-subunit interactions".

1991 – 1995: Medicine, Semipalatinsk State Medical Institute, Semipalatinsk, Kazakhstan.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Neurobiology of Learning and Memory | Sherman Building, North Wing | Tel Aviv University | Room: 521 | Phone: +972-3-6409821 | Email: This email address is being protected from spambots. You need JavaScript enabled to view it.