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Phone (office): +972-3-640-7633

Phone (Lab): +972-3-640-7536

Fax: +972-3-640-7499

Department of Molecular Microbiology and Biotechnology The George S. Wise Faculty of Life Sciences

Tel Aviv University

Tel Aviv 69978

Israel

 

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C. albicans and its human host

Candida species are the most prevalent human pathogens. They cause mucosal infections as well as life-threatening systemic infections. Here is shown a skin infecton of C. albicans.

Centromere Replication

C. albicans centromeres replicate first in the cell cycle which may be important for centromere inheritance. Neocentromeres also replicate first in each cell cycle. (Koren et al. 2010)

Aneuploidy/ploidy, loss of heterozygosity and stress

Aneuploidy (an imbalance in chromosome number) is detected in ~50% of fluconazole-resistant C. albicansisolates. Most isolates have an extra copy of one or more chromosomes. Whole genome ploidy changes may also occur under stress conditions.

C. albicans life cycle and parasexual cycle

C. albicans does not have a known meiotic cycle, but the diploids undergo parasex, in which diploids mate to form tetraploids. This occurs between diploids that are homozygous for the mating type-like locus and of opposite mating type and that have switched to a different physiological state, termed opaque. Parasexual progeny often are aneuploid for at least one chromosome and carry at least one homozygous chromosome. A proportion of them also have undergone multiple gene conversion events.  Thus, parasex can generate some genetic diversity, but not via the usual cross over events found in meiotic progeny. References: Forche et al. 2008 PLoS Biology Hadany and Berman 2012 Trends in Genetics Opaque switching in cells that are homozygous at the mating type locus Adapted from Berman and Hadany, Trends in Genetics 2012

SNP/CGH Arrays

SNP/CGH arrays allow detection of copy number variations, aneuploidies and loss of heterozygosity events Abbey at al. G3: Genes | Genomes | Genetics 2012

Judith Berman, Department of Molecular Microbiology and Biotechnology The George S. Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv 69978, Israel. +972-3-640-7633